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Responses of bacterial strains isolated from drinking water environments to N-acyl-L-homoserine lactones

Zhuoying WU, Qing WANG, Feng GUO, Shenghua ZHANG, Qipei JIANG, Xin YU

《环境科学与工程前沿（英文）》 2014年 第8卷 第2期   页码 205-214 doi: 10.1007/s11783-013-0492-5

摘要： Often as a result of biofilm formation, drinking water distribution systems (DWDS) are regularly faced with the problem of microbial contamination. Quorum sensing (QS) systems play a marked role in the regulation of microbial biofilm formation; thus, inhibition of QS systems may provide a promising approach to biofilm formation control in DWDS. In the present study, 22 bacterial strains were isolated from drinking water-related environments. The following properties of the strains were investigated: bacterial biofilm formation capacity, QS signal molecule N-acyl-L-homoserine lactones (AHLs) production ability, and responses to AHLs and AHL analogs, 3-chloro-4-(dichloromethyl)-5-hydroxy-2(5H)-furanone (MX) and 2(5H)-furanone. Four AHLs were added to developed biofilms at dosages ranging from 0.1?nmol·L to 100 nmol·L . As a result, the biofilm growth of more than 1/4 of the isolates, which included AHL producers and non-producers, were significantly promoted. Further, the biofilm biomasses were closely associated with respective AHLs concentrations. These results provided evidence to support the idea that AHLs play a definitive role in biofilm formation in many of the studied bacteria. Meanwhile, two AHLs analogs demonstrated unexpectedly minimal negative effects on biofilm formation. This suggested that, in order to find an applicable QS inhibition approach for biofilm control in DWDS, the testing and analysis of more analogs is needed.

关键词： drinking water distribution systems (DWDS)     biofilm     quorum sensing (QS)     N-acyl-L-homoserine lactones (AHLs)     (dichloromethyl)-5-hydroxy-2(5H)-furanone (MX)     2(5H)-furanone    

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Effects of exogenous acylated homoserine lactones on biofilms in biofilters for gaseous toluene treatment

《环境科学与工程前沿（英文）》 2023年 第17卷 第2期 doi: 10.1007/s11783-023-1617-0

摘要：

● Biofilm formation was enhanced by exogenous AHLs.

关键词： Quorum sensing     AHLs     Biofilter     Biofilm formation    

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Best operating conditions to produce hydroxyapatite nanoparticles by means of a spinning disc reactor

D’ Intino,B. de Caprariis,M.L. Santarelli,N. Verdone,A. Chianese

《化学科学与工程前沿（英文）》 2014年 第8卷 第2期   页码 156-160 doi: 10.1007/s11705-014-1427-9

摘要： In this work, Mg doped hydroxyapatite (Mg-HAP) nanoparticles were produced by a reaction-precipitation process by using a spinning disc reactor (SDR) at high rotational speed. The production process of these nanoparticles consisted of the neutralization reaction between two aqueous solutions of calcium chloride and ammonia orthophosphate at room temperature. By operating at pH= 10, a high purity Mg-HAP nanoparticles were obtained. In particular, they were 51 nm in average size when the two reagents were fed over the disc symmetrically at 3 cm from the disc center and a rotational speed of the disc reactor equal to 1400 r/min was adopted.

关键词： hydroxyapatite     nanoparticles     spinning disc reactor    

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Antifungal and antibacterial functions of medicinal leech recombinant destabilase-lysozyme and its heated-up derivative

YUDINA, Danyang GUO, N. F. PISKUNKOVA, I. B. PAVLOVA, L. L. ZAVALOVA, I. P. BASKOVA

《化学科学与工程前沿（英文）》 2012年 第6卷 第2期   页码 203-209 doi: 10.1007/s11705-012-1277-2

摘要： Antifungal activity of recombinant medicinal leech destabilase-lysozyme (rec.Dest-Lys) was investigated by using fungi: , and , including yeasts and . Its antibacterial activity was investigated on gram-negative bacteria . These activities were assessed by radial agar diffusion assay, and scanning and transmission electron microscopy. Therefore, destabilase-lysozyme not only is endo-isopeptidase and lysozyme, but also has antifungal and antibacterial activities. Muramidase activity of rec.Dest-Lys disappeared after heat-treating at 90°C for 50 min without the loss of its antimicrobial activity. Furthermore we showed for the first time that the heated-up derivative of rec.Dest-Lys exhibited more potent activities against the above enumerated fungi and gram-negative bacteria than original protein.

关键词： recombinant destabilase-lysozyme (rec.Dest-Lys)     antimicrobial activity     antifungal activity     medicinal leech     heated-up derivative of rec.Dest-Lys (T-rec.Dest-Lys)    

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Simultaneous harvesting and cell disruption of microalgae using ozone bubbles: optimization and characterization study for biodiesel production

Wan N. A. Kadir, Man K. Lam, Yoshimitsu Uemura, Jun W. Lim, Peck L. Kiew, Steven Lim, Siti S.Wong, Pau L. Show, Keat T. Lee

《化学科学与工程前沿（英文）》 2021年 第15卷 第5期   页码 1257-1268 doi: 10.1007/s11705-020-2015-9

摘要： In the present study, ozone was introduced as an alternative approach to harvest and disrupt microalgae cells ( ) simultaneously for biodiesel production. At the optimum ozonation conditions (6.14 g·h ozone concentration, 30 min ozonation time, 1 L·min of ozone flowrate at medium pH of 10 and temperature of 30 °C), the sedimentation efficiency of microalgae cells increased significantly from 12.56% to 68.62%. It was observed that the microalgae cells aggregated to form flocs after pre-treated with ozone due to the increment of surface charge from –20 to –6.59 mV. Besides, ozone had successfully disrupted the microalgae cells and resulted in efficient lipid extraction, which was 1.9 times higher than the control sample. The extracted microalgae lipid was mainly consisted of methyl palmitate (C16:0), methyl oleate (C18:1) and methyl linolenate (C18:3), making it suitable for biodiesel production. Finally, utilization of recycled culture media after ozonation pre-treatment showed robust growth of microalgae, in which the biomass yield was maintained in the range of 0.796 to 0.879 g·h for 5 cycles of cultivation.

关键词： microalgae     biodiesel     ozonation     pre-treatment     lipid    

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Kinematic calibration of precise 6-DOF stewart platform-type positioning systems for radio telescope applications

HERNáNDEZ, Marco CECCARELLI, Carlos LóPEZ-CAJúN, Alejandro GARCíA

《机械工程前沿（英文）》 2013年 第8卷 第3期   页码 252-260 doi: 10.1007/s11465-013-0249-7

摘要：

The pose accuracy of a parallel robot is a function of the mobile platform posture. Thus, there is no a single value of the robot’s accuracy. In this paper, two novel methods for estimating the accuracy of parallel robots are presented. In the first method, the pose accuracy estimation is calculated by considering the propagation of each error, i.e., error variations are considered as a function of the actuator’s stroke. In the second method, it is considered that each actuator has a constant error at any stroke. Both methods can predict pose accuracy of precise robots at design stages, and/or can reduce calibration time of existing robots. An example of a six degree-of-freedom parallel manipulator is included to show the application of the proposed methods.

关键词： pose errors     error estimation     parallel robot     radio telescopes    

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Association between heavy metals and antibiotic-resistant human pathogens in environmental reservoirs: A review

Nguyen, Cody N. Hugie, Molly L. Kile, Tala Navab-Daneshmand

《环境科学与工程前沿（英文）》 2019年 第13卷 第3期 doi: 10.1007/s11783-019-1129-0

摘要：

Heavy metals can act as co-selecting agents and promote antibiotic resistance.

Most frequent resistances to heavy metals are observed for zinc and cadmium.

P. aeruginosa and E. coli are commonly resistant to heavy metals and antibiotics.

Heavy metals proliferate antibiotic resistance through co- and cross-resistance.

Heavy metal and antibiotic resistances are common near anthropogenic activities.

关键词： Zinc     Cadmium     Copper     Lead     E. coli     P. aeruginosa    

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Wide gap active brazing of ceramic-to-metal-joints for high temperature applications

Bobzin,L. Zhao,N. Kopp,S. Samadian Anavar

《机械工程前沿（英文）》 2014年 第9卷 第1期   页码 71-74 doi: 10.1007/s11465-014-0291-0

摘要：

Applications like solid oxide fuel cells and sensors increasingly demand the possibility to braze ceramics to metals with a good resistance to high temperatures and oxidative atmospheres. Commonly used silver based active filler metals cannot fulfill these requirements, if application temperatures higher than 600°C occur. Au and Pd based active fillers are too expensive for many fields of use. As one possible solution nickel based active fillers were developed. Due to the high brazing temperatures and the low ductility of nickel based filler metals, the modification of standard nickel based filler metals were necessary to meet the requirements of above mentioned applications. To reduce thermally induced stresses wide brazing gaps and the addition of Al2O3 and WC particles to the filler metal were applied. In this study, the microstructure of the brazed joints and the thermo-chemical reactions between filler metal, active elements and WC particles were analyzed to understand the mechanism of the so called wide gap active brazing process. With regard to the behavior in typical application oxidation and thermal cycle tests were conducted as well as tensile tests.

关键词： wide gap     active brazing     nickel filler metals     high temperature application     WC     Al2O3    

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Experimental evaluation and simulation of volumetric shrinkage and warpage on polymeric composite short natural fibers reinforced injection molded

GARZÓN,Luis M. LÓPEZ

《机械工程前沿（英文）》 2015年 第10卷 第3期   页码 287-293 doi: 10.1007/s11465-015-0346-x

摘要：

A polymeric natural fiber-reinforced composite is developed by extrusion and injection molding process. The shrinkage and warpage of high-density polyethylene reinforced with short natural fibers of Guadua angustifolia Kunth are analyzed by experimental measurements and computer simulations. Autodesk Moldflow® and Solid Works® are employed to simulate both volumetric shrinkage and warpage of injected parts at different configurations: 0 wt.%, 20 wt.%, 30 wt.% and 40 wt.% reinforcing on shrinkage and warpage behavior of polymer composite. Become evident the restrictive effect of reinforcing on the volumetric shrinkage and warpage of injected parts. The results indicate that volumetric shrinkage of natural composite is reduced up to 58% with fiber increasing, whereas the warpage shows a reduction form 79% to 86% with major fiber content. These results suggest that it is a highly beneficial use of natural fibers to improve the assembly properties of polymeric natural fiber-reinforced composites.

关键词： biocomposite     natural fiber     shrinkage     simulation     warpage    

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Seismic performance of composite moment-resisting frames achieved with sustainable CFST members

JIANG,L. MACEDO,J. M. CASTRO,R. MONTEIRO,N. SILVESTRE

《结构与土木工程前沿（英文）》 2016年 第10卷 第3期   页码 312-332 doi: 10.1007/s11709-016-0345-y

摘要： The main objective of the research presented in this paper is to study the bending behaviour of Concrete Filled Steel Tube (CFST) columns made with Rubberized Concrete (RuC), and to assess the seismic performance of moment-resisting frames with these structural members. The paper describes an experimental campaign where a total of 36 specimens were tested, resorting to a novel testing setup, aimed at reducing both the preparation time and cost of the test specimens. Different geometrical and material parameters were considered, namely cross-section type, cross-section slenderness, aggregate replacement ratio, axial load level and lateral loading type. The members were tested under both monotonic and cyclic lateral loading, with different levels of applied axial loading. The test results show that the bending behaviour of CFST elements is highly dependent on the steel tube properties and that the type of infill does not have a significant influence on the flexural behaviour of the member. It is also found that Eurocode 4 is conservative in predicting the flexural capacity of the tested specimens. Additionally, it was found that the seismic design of composite moment-resisting frames with CFST columns, according to Eurocode 8, not only leads to lighter design solutions but also to enhanced seismic performance in comparison to steel frames.

关键词： concrete filled steel tubes     rubberized concrete     composite frames     seismic performance assessment    

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Synthesis of a cardanol-amine derivative using an ionic liquid catalyst

Trevisan, Roseane L. E. da Silva, Roselayne F. Furtado, Zengshe Liu, H. N. Cheng

《化学科学与工程前沿（英文）》 2016年 第10卷 第3期   页码 425-431 doi: 10.1007/s11705-016-1581-3

摘要： Cardanol is a biobased raw material derived from cashew nut shell liquid. In order to extend its utility, new derivatives and additional applications are useful. In this work cardanol was first epoxidized, and a novel aniline derivative prepared from it under mild reaction conditions with the help of an ionic liquid catalyst. The reaction chemistry was studied by using nuclear magnetic resonance. The resulting aminohydrin adduct showed antioxidant property and should also be a useful synthon for further reactions. As an example, the aminohydrin was shown to undergo a condensation reaction with formaldehyde to form a prepolymer, which could be further reacted to form thermosetting resins.

关键词： cardanol     epoxidation     aminohydrin     aniline     ionic liquid     aniline-formaldehyde    

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Application of StrucGP in medical immunology: site-specific -glycoproteomic analysis of macrophages

《医学前沿（英文）》 2023年 第17卷 第2期   页码 304-316 doi: 10.1007/s11684-022-0964-8

摘要： The structure of N-glycans on specific proteins can regulate innate and adaptive immunity via sensing environmental signals. Meanwhile, the structural diversity of N-glycans poses analytical challenges that limit the exploration of specific glycosylation functions. In this work, we used THP-1-derived macrophages as examples to show the vast potential of a N-glycan structural interpretation tool StrucGP in N-glycoproteomic analysis. The intact glycopeptides of macrophages were enriched and analyzed using mass spectrometry (MS)-based glycoproteomic approaches, followed by the large-scale mapping of site-specific glycan structures via StrucGP. Results revealed that bisected GlcNAc, core fucosylated, and sialylated glycans (e.g., HexNAc4Hex5Fuc1Neu5Ac1, N4H5F1S1) were increased in M1 and M2 macrophages, especially in the latter. The findings indicated that these structures may be closely related to macrophage polarization. In addition, a high level of glycosylated PD-L1 was observed in M1 macrophages, and the LacNAc moiety was detected at Asn-192 and Asn-200 of PD-L1, and Asn-200 contained Lewis epitopes. The precision structural interpretation of site-specific glycans and subsequent intervention of target glycoproteins and related glycosyltransferases are of great value for the development of new diagnostic and therapeutic approaches for different diseases.

关键词： macrophage     glycoproteome     glycopeptides     N-glycan structures     PD-L1    

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适用于低轨微小卫星的大视场红外地球敏感器

王昊1,王志远1,王本冬1,金仲和1,John L. CRASSIDIS2

《信息与电子工程前沿（英文）》 2021年 第22卷 第2期   页码 141-286 doi: 10.1631/FITEE.1900358

摘要： 红外地球敏感器广泛应用于卫星姿态确定和控制系统中。静态红外地球敏感器的主要缺点是视场较小，其典型视场约为20°至30°，可能不适用于近地轨道的微型卫星。设计了一种新型的小型红外地球敏感器，其视场接近180°。该地球敏感器包括全景环形透镜（PAL）和红外CMOS传感器。PAL镜头用于增强视场，从而从近地轨道获得完整的地球红外图像，据此提出一种补偿PAL引起的畸变和计算地球矢量的算法。新型红外地球敏感器结构紧凑，功耗低，精度高。利用ZDPS-2微小卫星获得在轨红外图像和模拟图像，评估新型红外地球敏感器的性能，实验表明其精度约为0.032°。

关键词： 红外地球敏感器；微小卫星；姿态确定系统    

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ACSL5, a prognostic factor in acute myeloid leukemia, modulates the activity of Wnt/-catenin signaling by palmitoylation modification

《医学前沿（英文）》 2023年 第17卷 第4期   页码 685-698 doi: 10.1007/s11684-022-0942-1

摘要： Acyl-CoA synthetase long chain family member 5 (ACSL5), is a member of the acyl-CoA synthetases (ACSs) family that activates long chain fatty acids by catalyzing the synthesis of fatty acyl-CoAs. The dysregulation of ACSL5 has been reported in some cancers, such as glioma and colon cancers. However, little is known about the role of ACSL5 in acute myeloid leukemia (AML). We found that the expression of ACSL5 was higher in bone marrow cells from AML patients compared with that from healthy donors. ACSL5 level could serve as an independent prognostic predictor of the overall survival of AML patients. In AML cells, the ACSL5 knockdown inhibited cell growth both in vitro and in vivo. Mechanistically, the knockdown of ACSL5 suppressed the activation of the Wnt/β-catenin pathway by suppressing the palmitoylation modification of Wnt3a. Additionally, triacsin c, a pan-ACS family inhibitor, inhibited cell growth and robustly induced cell apoptosis when combined with ABT-199, the FDA approved BCL-2 inhibitor for AML therapy. Our results indicate that ACSL5 is a potential prognosis marker for AML and a promising pharmacological target for the treatment of molecularly stratified AML.

关键词： acute myeloid leukemia     acyl-CoA synthetase long chain family member 5     Wnt3a     palmitoylation     ABT-199    

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Comments on the special issue on forestry of

John L. INNES

《农业科学与工程前沿（英文）》 2017年 第4卷 第4期   页码 502-503 doi: 10.15302/J-FASE-2017189

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标题 作者 时间 类型 操作

Responses of bacterial strains isolated from drinking water environments to N-acyl-L-homoserine lactones

Zhuoying WU, Qing WANG, Feng GUO, Shenghua ZHANG, Qipei JIANG, Xin YU

期刊论文

Effects of exogenous acylated homoserine lactones on biofilms in biofilters for gaseous toluene treatment

期刊论文

Best operating conditions to produce hydroxyapatite nanoparticles by means of a spinning disc reactor

D’ Intino,B. de Caprariis,M.L. Santarelli,N. Verdone,A. Chianese

期刊论文

Antifungal and antibacterial functions of medicinal leech recombinant destabilase-lysozyme and its heated-up derivative

YUDINA, Danyang GUO, N. F. PISKUNKOVA, I. B. PAVLOVA, L. L. ZAVALOVA, I. P. BASKOVA

期刊论文

Simultaneous harvesting and cell disruption of microalgae using ozone bubbles: optimization and characterization study for biodiesel production

Wan N. A. Kadir, Man K. Lam, Yoshimitsu Uemura, Jun W. Lim, Peck L. Kiew, Steven Lim, Siti S.Wong, Pau L. Show, Keat T. Lee

期刊论文

Kinematic calibration of precise 6-DOF stewart platform-type positioning systems for radio telescope applications

HERNáNDEZ, Marco CECCARELLI, Carlos LóPEZ-CAJúN, Alejandro GARCíA

期刊论文

Association between heavy metals and antibiotic-resistant human pathogens in environmental reservoirs: A review

Nguyen, Cody N. Hugie, Molly L. Kile, Tala Navab-Daneshmand

期刊论文

Wide gap active brazing of ceramic-to-metal-joints for high temperature applications

Bobzin,L. Zhao,N. Kopp,S. Samadian Anavar

期刊论文

Experimental evaluation and simulation of volumetric shrinkage and warpage on polymeric composite short natural fibers reinforced injection molded

GARZÓN,Luis M. LÓPEZ

期刊论文

Seismic performance of composite moment-resisting frames achieved with sustainable CFST members

JIANG,L. MACEDO,J. M. CASTRO,R. MONTEIRO,N. SILVESTRE

期刊论文

Synthesis of a cardanol-amine derivative using an ionic liquid catalyst

Trevisan, Roseane L. E. da Silva, Roselayne F. Furtado, Zengshe Liu, H. N. Cheng

期刊论文

Application of StrucGP in medical immunology: site-specific -glycoproteomic analysis of macrophages

期刊论文

适用于低轨微小卫星的大视场红外地球敏感器

王昊1,王志远1,王本冬1,金仲和1,John L. CRASSIDIS2

期刊论文

ACSL5, a prognostic factor in acute myeloid leukemia, modulates the activity of Wnt/-catenin signaling by palmitoylation modification

期刊论文

Comments on the special issue on forestry of

John L. INNES

期刊论文